The porcine brucellosis--evidence of the role of Yersinia enterocolitica O:9 in occurrence of false positive serological reactions.
نویسندگان
چکیده
Forty four pigs with typical characteristics for false positive serolpgical reactions (FPSR) were examined for the presence of Yersinia enterocolitica O:9. The positive reactions were observed in rose bengal test (RBT, N = 23 sera), serum agglutination test (SAT, N = 16), complement fixation test (CFT, N = 9), indirect ELISA (i-ELISA, N= 11) in first, and in RBT (N = 14), SAT (N = 8), CFT (N = 7) and i-ELISA (N = 18) in second examination, respectively. In bacteriological examination Y enterocolitica was confirmed in 12 cases. Six of these isolates were identified with PCR as Y enterocolitica 0:9.
منابع مشابه
Specificity dependence between serological tests for diagnosing bovine brucellosis in Brucella-free farms showing false positive serological reactions due to Yersinia enterocolitica O:9.
When brucellosis false positive serological reactions happen in cattle, the serial use of pairs of specificity-correlated serological tests (rose bengal, complement fixation, competitive ELISA) results in specificities lower than expected. In this situation, highly specific tests, such as the indirect ELISA used alone, may be more adequate than serial testing.
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عنوان ژورنال:
- Polish journal of veterinary sciences
دوره 16 1 شماره
صفحات -
تاریخ انتشار 2013